بررسی باززایی مستقیم شاخساره از قطعات جدا کشت ساقه سیب زمینی دلوار توسط هورمون (Thidiazuron) TDZ

Potato is a valuable plant as a source of nutrition and as an industrial product. The modification and breeding of potato plants are very difficult, or even impossible, via the conventional plant breeding programs. However, plant biotechnology and tissue culture are reliable methods which can produce plant material for gene transformation using Agrobacterium as a vector. In this study, one-, two-, and three-step methods of plant regeneration from stem culture of potato cv. Delaware were tested. Among these only the one-step procedure using TDZ, a synthetic cytokinin, was the best for rapid plant regeneration. In this culture medium, several shoot buds and shoots were regenerated from stem culture, while the other methods using a culture medium supplemented with BAP, NAA, zeatin, 2ip, IAA produced white and green callus. Morphology and chromosome number of all regenerated plants were similar to the original plants. The results showed that the regeneration system was suitable for cv. Delaware and that the culture conditions prevented genetic variation of the regenerated plants.